Template selection by E. Coli RNA polymerase holoenzyme
نویسندگان
چکیده
منابع مشابه
Template specific inhibitors of E. coli RNA polymerase.
Electroneutral analogs of polynucleotides, poly-9-vinyladenine and poly-1-vinyluracil inhibit E. coli RNA polymerase in all combinations where the single stranded polynucleotides are used as templates and the vinyl analogs are complementary to them. As templates both the ribo- and deoxyribopolynucleotides were tested; variation of the template concentration in the presence of vinyl analogs prod...
متن کاملStructure of Escherichia coli RNA polymerase holoenzyme at last.
Much of the mechanistic foundations of our knowledge of regulation of gene expression at the transcriptional level have been provided by Escherichia coli and its phages. E. coli RNA polymerase (EcoRNAP) is a multisubunit enzyme composed of a catalytically active core (β′βα2ω); subunits that are evolutionarily related to β′, β, α, and ω are present in DNA-dependent RNAPs of all organisms. In bac...
متن کاملDNA polymerase III holoenzyme of Escherichia coli.
DNA polymerase III holoenzyme has been purified from Escherichia coli HMS-83, using, as an assay, the conversion of coliphage G4 single-stranded DNA to the duplex replicative form. The holoenzyme consists of at least four different subunits: a, /I, y, and 6 of 140,000, 40,000, 52,000, and 32,000 daltons, respectively. The (Y subunit is DNA polymerase III, the dnaE gene product. The holoenzyme h...
متن کاملThe kinetics of E. coli RNA polymerase.
Using an assay specific for chain elongation of E. coli RNA polymerase the kinetics of this propagation reaction have been studied. The kinetic behaviour is consistent woth the mathematical model formulated for this multisubstrate enzyme. The effect of increasing salt concentration on the kinetics of the reaction indicated that DNA unwinding is probably a necessary step in the propagation step,...
متن کاملCrl facilitates RNA polymerase holoenzyme formation.
The Escherichia coli Crl protein has been described as a transcriptional coactivator for the stationary-phase sigma factor sigma(S). In a transcription system with highly purified components, we demonstrate that Crl affects transcription not only by the Esigma(S) RNA polymerase holoenzyme but also by Esigma(70) and Esigma(32). Crl increased transcription dramatically but only when the sigma con...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1976
ISSN: 0014-5793
DOI: 10.1016/0014-5793(76)80685-0